Abstract:
A simple HPLC method was developed and validated for estimation of mefenamic acid, using UV-Visble detector at 275 nm on a reverse phase column (phenomenx, C18 4.6 mm × 2.5 mm), with mobile phase consisting of: acidified water (pH 3.2 adjusted With ortho phosphoric acid) and methanol ( 10:90 v/v) pumped at a flow rate of 1 ml/min at retention time ~ 3.5 min with symmetrical peaks. Mefnamic acid was detected by ultraviolet absorbance at 275 nm 20 µƖ with no interference of commonly used excipient.
The validation parameters (selectivity, linearity, precision, accuracy and quantification limit) were measured. The Linearity shows straight line in the range: 5-25 µ g /Ɩ, with coefficients (R2) = 0.999 and the Relative standard deviation % were found to be less than 2% for three replicates samples. The recoveries obtained ranged from 102 - 104 %. The proposed method is rapid, accurate and selective.
