Abstract:
Diarrheagenic Escherichia coli is one of the most important etiologic agents
of several infections, and represent a major public health problem in
developing countries like Sudan. The present study was undertaken to detect
the virulence factors of diarrheagenic E. Coli strains using multiplex PCR in
isolated samples of urine, high vaginal swab and wound swab. Fifty clinical
isolate were collected from Omdurman military hospital and police hospital in
Khartoum State; they were from 21(42%) males and 29(58%) females. Then
they cultured on MacConkey agar and EMB media. Then biochemical tests
were done, including indole test, citrate, Kilgelar iron agar and urease test.
The sensitivity test was performed using Disc diffusion method; the following
antibiotic discs were used (Ceftriaxone, Meropenem, Imipenem, Amikacin,
Ceftazidime, Ciprofloxacin Gentamicin and Framycetin). Most of the isolated
organisms were resistant to these antibiotics. DNA was extracted by boiling
method. Multiplex PCR techniques was used to amplify four genes of
diarrheagenicE.coli, the frequency of Enteroaggregative E.coli (aggRgene)
was found in 8 (16) % in urine isolate , and negative in other isolates,
Enterohermorrhagic E.coli (stxgene)was2(4%) in urine isolates and negative
in other isolates,Enteroinvasive E.coli (ipaH gene) was (8) 16 % in urine
isolate, and 2 (4%) in wound swab and 1(2%) in High vaginal swab.
Enteropathogenic E.coli(eae gene) was negative in all isolates. From this
study we concluded that, the presence of diarrheagenic E.coliin other samples
than stools may indicate the source of infection and the predominant strain is
Enteroinvasive E.coli .
