Frequency of Helicobacter.pylori ureA gene From Fecal Specimens using (Polymerase chain reaction)

Abstract

This study aimed to detect Helicobacter pylori ureA gene in fecal specimen using PCR among patient attended to Saad Rishwan health center, Omdurman, from May, 2017 to March 2018. Personal and clinical data were collected by questionnaire after verbal consent, stool samples were collected.immenochrometography test for stool Helicobacter pylori antigen was performed, then polymerase chain reaction was used to detect ureA gene It was found that 37(61.7%),23(38.3%) are positive to ICT for female and male respectively . Out of 60 patients positive for H.pylori stool Ag ,7 (11. 7%) of them were positive for PCR . This study concluded that detection of H.pylori using fecal specimens Without removal of inhibiters may give false negative result And the use of ureA primers for detection of H.pylori may be less sensitive than others genes. Also the high sensitivity of H.pylori stool antigen test my give false positive.