Abstract:
This is analytical case-control study carried out in Khartoum state during the
period from December 2009 to March 2010, the study aimed at determing the
best fixative agent for the urine samples. Specimens were taken from
cigarettes
smokers
for
identification
of
cellular
changes
by
using
Papanicolaou method. Three hundred urine smears were collected randomly
from men. Of whom150 were continually exposed to cigarette smoking for
more than 10 years and 10 cigarettes per day, and the remaining 150 were
taken from apparently healthy individuals (non tobacco users).
The cytological assessment among study group revealed the following
findings: fixation with 95% ethyl alcohol found that, the mean of nucleus stain
was 3.4800 and 3.6333 for cytoplasm stain. In cytoRich fixative the mean of
nucleus stain was 5.9433 and for cytoplasm stain was 5.9533. Esposti's
fixative was the higher value for mean with 6.4433 for nucleus stain and
6.4067 for cytoplasm stain. In regard to the cytological changes, acute
inflammatory changes were detected in (38) of smokers, and (6) of non-
tobacco users, and this was found to be statistically highly significant (P.
<0.0001).
In conclusion of this study, the Esposti's fixative is a good fixative and
cytoRich fixative was moderate fixative and 95% ethyl alcohol was poor
fixative for the urine cells. Also the study found relation between smoking and
acute inflammatory cells in urine.
