Abstract:
In this work, a measurement of the concentration for (Albumin,
Urea, Phosphorous, Calcium, and Total protein.) in human blood is
obtained using the He-Ne laser and compared to results that achieved by
the conventional Spectrophotometric methods .
To ensure the possibility of absorbing the wavelength 632.8nm by
the mentioned blood components, a spectral analysis was done with 1240
UV-Vis Spectrophotometer. The results showed absorbing peaks in the
range (600-700)nm, thus a laser system consist of He-Ne laser (with
power 1mW, and wavelength 632.8nm, sample chamber, Quartz cuvett,
and detection element (Photomultiplier tube) joined with digital
multimeter was prepared to measure the concentrations.)
The tested samples were prepared in the laboratory, its
concentration is measured by the laser system immediately within its
colored stability period after the measurement that done by
spectrophotometer.
A comparison between the concentrations obtained by the two
methods was done statistically by calculating the linear correlation
coefficient and plotting the standard curves between absorbance and
concentration.
The compared results showed the possibility of determining the
concentrations of (Albumin, Urea, Phosphorous, Calcium, and Total
Protein); by using the He-Ne laser absorption spectroscopy technique
with high accuracy and least cost from that determined by the
Spectrophotometer.
