Detection of Helicobacter pylori in Stool Specimens using Immunochromatographic Assay and Polymerase Chain Reaction in Saad Rshwan Hospital

Abstract

Helicobacter pylori is a microaerophlic, Gram negative, motile, curved rod, which inhabits the gastric mucosa of the human stomach. The organism chronically infects billions of people world wide. Infections with organism potentially induces chronic gastritis and peptic ulcer disease. In a ddtion H. pylori a role in the etiology of gastric cancer and gastric MALT lymphoma. In this cross sectional study, the aim of present study to detect H. pylori in patients stool specimens using immunochromatography test and polymerase chain reaction among patients attending Saad Rshwan Hospital at time of specimens collection. Faecal samples were collected from 50 patients suffering from dyspeptics, consisting of 20 (40%) males, 30 (60%) females aged from 10 years to 85 years (mean = 32.24, SD = 17.6). H. pylori antigen rapid test immunochromatography were used to analyze the faecal samples for detection H. pylori antigen in stool. Among 50 faecal samples positive for H. pylori antigen, also feacal samples DNA were extracted by iNtron stool genomic DNA extraction mini kit, and by conventional polymerase chain reaction targeting the ure A gene in H. pylori was carried out to detect H. pylori DNA in feacal samples of already positive feacal samples by H. pylori ICT. Chisquare statistical analysis was used to determine p.value (0.05) significance range. Twenty nine (58%) of fifty feacal samples that had previously tested positive for the organism by ICT H. pylori antigen were confirmed positive by PCR, 10 (20%) males, 19(38%) females. But the associations between the ure A gene of H. pylori, age groups (p. value=0.8), gender (p. value=0.7), educations level (p. value=0.9), marital status (p. value=0.8), family history (p. value=0.4) and smoking behavier (p. value=0.5) of patients were not significant, not reach the significant range 0.05. The present study revealed ahigh frequency of H. pylori in feacal samples, and the PCR may be more accurate in the diagnosis. Further work is needed to validate these results.