Detection of Helicobacter pylori CagA Gene in Gastric Lesions among Sudanese Gastric Disorder Patients

Abstract

This descriptive case study was conducted at Faisal special hospital , Fedail hospital and Omdurman military hospital during the period from April to December 2018. The aim of this study was to detect Helicobacter pylori CagA gene in patients suffering from gastric disorder using polymerase chain reaction (PCR). A total of 38 (n=38) of both sexes were included for this study, patient data were collected by questionnaire .female were 21(55.3%), male were17 (44.7%) and ages ranges from 16 to 80 year, with mean age 56. Endoscopic biopsy samples were collected, processed and stained by H&E method for histo patholigical changes, and PCR for detection CagA gene for H.pylori. Histopatholog results were shown the following: inflammatory changes in 25(65.8%) samples, dysplasia in only one sample (2.6)% and no changes in 12(31.6)samples. The result of PCR for CagA gen were shown as follow, 30(78.9) samples were positive and 8(21.1%) samples were negative. The study conclude there is no association between and gastric changes.