Immunohistochemical Detection of Estrogen Receptors in Ovarian Tumors in Sudanese Women

Abstract

This is analytical retrospective case controlhospital based study was conducted at Alamal Hospital and Altayseer-2 labrotary in Khartoum state during the period from April 2018 to August 2018. The studyaimed to detect estrogen receptor expression in ovarian tumors using immunohistochemistry. Fifty seven paraffin blocks were collected from patients samples previously diagnosed as ovarian tumors, 40 (66.6%) samples were malignant and 17 (33.4%) samples were benign. The histopathological subtype of malignant samples includes 24 (40%) serous adenocarcinoma, 5 (8.3%) mucinous adenocarcinoma, 8 (13.3%) granulosa cell tumors and 3 (5%) non epithelial tumor. One section of 3μm was cut from each paraffin block, then stained by immunohistochemical method (new indirect technique) for detection of ER expression. The data obtained was analyzed using SPSS program version 16. The age of studypopulation range between 28 and 70 years with mean age of 49 years. Most of patients were younger than 50 years representing 32(56%) and the remaining 25(44%) were older than 50 years. ER expression was revealed positive result in 3 (7.5%) samples in malignant tumor while 37 (92.5%) samples showed negative expression, In benign ovarian tumors positive expression was found in 2 (11.7%) samples and 15(88.3%) samples showed negative expression, this result showed insignificant association between ER expression and malignant ovarian tumors (P. value =0.27). The study conclude that no association between ER expression and types of ovarian tumors.