Detection of Methylene Tetra Hydro Folate Reductase Gene C677T Polymorphism and Coagulation Tests among Sudanese patients with Deep Vein Thrombosis

Abstract

This is analytical case control study designed to investigate the relationship between methylene tetra hydro folate reductase MTHFR gene (C677T) polymorphism and deep vein thrombosis in Khartoum state in the period between February, 2018 and January, 2019 .this study included Forty Sudanese patients who previously diagnosed as DVT (14 male 35% and 26 female 65%) compared with Forty healthy people as control (30 male 75% and 10 female 25%).The mean age of case group was 38.08, age range of 21- 40 years, mean age of control group was 29.03, age range 21-40 years. The mean of disease duration in month in study group was 12.13, duration range of 1-12 months. 31 (77.5%) of case group was use heparin as treatment and 9 (22.5%) of case group was use warfarin. The variable frequencies of case group under study included: pregnancy 46.2%, contraceptive pills 84.6% and surgery32.5%. Blood samples were collected from participants in EDTA and tri sodium citrate anticoagulant containers after filling the questionnaire. Then DNA was extracted by using guanidine chloride method, and the frequency of the gene polymorphism in the patients and controls were determined using PCR-restriction fragment length polymorphism, then followed by PT and PTT measurement by coagulometer instrument. The results were analyzed by statistical package for social science (SPSS) computer program. The result showed that all the patients confirmed to have DVT had the wild gene and hence were negative for MTHFR (C677T) gene mutation. There were no positivity for mutation among control group. The result also showed that the mean of prothrombin time (PT) and partial thromboplastin time (PTT) of case group are no significantly different than that of control group (P.value 0.321 and P.value 0.098 reapectively).