Detection of Coagulation Factor XIII-A (Val34Leu) Polymorphism in Sudanese Patients with Deep Vein Thrombosis

Abstract

This is analytical case- control study was conducted among Sudanese DVT patients to detect the most common polymorphism of coagulation factor XIII-A (Val34Leu), the practical was performed in the research laboratory of Sudan university of sciences and technology in period between March and July 2018. Eighty venous blood samples (2.5 ml) were collected in EDTA containers, forty samples from patients were already diagnosed with the DVT by imaging of dopplar ultra sonography, patients with different ages and both gender were included, some risk factors such as hypertension, pregnancy and contraceptives, obesity and smoking were considered, and forty samples were collected from healthy individuals as controls, all samples were kept in -20°C for DNA extraction. DNA was extracted by guanidine hydrochloride method, PCR were performed on the eighty samples and the presence of factor XIII- A (val34leu) polymorphism was determined by the use of restriction enzyme. Four polymorphisms were detected in the patient samples, 3 were heterozygous (val/leu) and one was homozygous (leu/leu), and no polymorphism was detected in the control samples. These results were analyzed by statistical package for social sciences computer programme (SPSS), the p-value was insignificant and the study |concluded that no association between factor XIII-A (val34leu) polymorphism and DVT.