In Vitro UV-Visible and FTIR Spectroscopy Study of Low Power He-Ne Laser Irradiation on Human Blood

Abstract

Low-power helium-neon laser recently has been used numerously in medical applications. FTIR and UV-Vis spectroscopic technique is employed to study the spectral differences in the serum of whole blood samples. Human Whole Blood was Irradiated to (He-Ne) laser (λ= 632 nm, power =2 mW) and (He-Ne) laser (λ= 632 nm, power = 1mW) with different times from 10 min to 100 min. Blood samples were collected from healthy volunteers; and exposed to (He-Ne) laser and compared with control. UV-Vis spectrophotometer and FTIR were used to study the effect of laser radiation. FTIR spectrum of non exposed blood showed the peaks due to O-H (free group), C=O (amide I group), N=O (nitro group), and C-H (aromatic group). N-H (Amino acid (amide II) for all exposure times He-Ne laser (λ= 632nm, power=1mW) irradiation, showed significant changes. Increased in transmittance was observed at different exposure times for all groups (C=O, O-H, N=O, C-O & C-H, N-H) and indicates significant decreasing in their concentration. The most effects are found when whole blood was irradiated to He-Ne laser radiation power 2mW for 10 and 20 min and transmittance decreases for C-H, and He-Ne, while transmittance increase for all groups at rest of exposure times. Blood irradiated to He-Ne laser radiation power 1mW for 80 min show decrease of transmittance for groups (N=O, C-H, N-H, C-O, O-H) but an increase for group C=O only is observed. Photodegradation of blood components due to absorption of laser radiation causes changes in the structure and conformational changes in the polypeptide. Human Whole Blood Irradiated to (He-Ne) laser (λ= 632 nm, power =2 mW shows, in Uv-Visible spectra a significant decrease in intensity. Result showed Blood Irradiated to (He-Ne) laser, power =1 mW absorption intensity decreases for all exposure time except at (10, 40 and 70 min), this fluctuation of light absorption is known as a biphasic response.