Microbiological Investigation of Helicobacter pylori in Faeces by Culture and Polymerase Chain Reaction among Patients with Gastrointestinal Symptoms in Omdurman locality

Abstract

The aim of this study was to detect of Helicobacter pylori (H. pylori) in faeces by culture and Polymerase Chain Reaction among patients with gastrointestinal symptoms in relation to selected risk factors in Omdurman locality. This was cross sectional case control study. The study was conducted fifty symptomatic patients (n=50)19 were males and 31 were female. The age of patients ranging from 10 –85 years. Sociodemographic data were collected from patient by using structured questionnaire. All stool samples were cultured on selective Columbia blood agar media for isolation. DNA extraction by i-genomic Stool DNA Extraction Mini Kit and multiplex PCR were applied to these samples to detect H.pylori Urea A gene. The results showed that out of 50 samples, no growth was seen on culture media. The prevalence of H. pylori according to Polymerase chain reaction is 58% . 20% were males and 38% were females which is statstically insignificant ( p = 0.547). Statistical analysis showed that there was insignificant correlation between H.pylori infection and gender , age , social status , gastrointestinal symptoms , educational level , family history of infection with H. pylori , smoking behavior , source of water (P >0.05).