Determination of Platelets Counts and Indices,Hemoglobin Electrophoresis and Sickling Test among Sickler Patients in Khartoum State

Abstract

This is case-control analytical study that aimed to determine platelet count and indices , also to detect hemoglobin electrophoresis and sickling test in sickle cell patients in Khartoum state. This study was conducted during September-2018 to April-2019 in Khartoum state. following informed consents, one hundred person were included (fifty were cases (26 males and 24 females) and fifty were controls (25 males and 25 females)blood samples were taken from both group at Alzaytouna Specialist Hospital. Two ml of venous blood were collected from hundred individuals into EDTA containers. All samples were tested for platelets count and indices, sickling test and hb electrophoresis. The blood samples were tested by sysmex (kx-21N), a fully automated device and Mini cap Cibia for Hb electrophoresis, all results were analyzed by (SPSS) program. The frequency of sickle cell anemia showed that there were 26 individuals (52%) sickle cell trait (hb-AS), and 24 individuals (48%) were sickle cell disease (hb-SS). The results showed that platelets counts and platelet indices (MPV , PDW and PCT) were higher in case compared with controls(P:0.000), (P:0.000), (P:0.005) and (P:0.000) respectively. but the platelets indices P-LCR (P:0.666) difference were not changed. Mean of plt count, MPV, PDW, PCT and P-LCR were (425.64 ± 171.250), (10.250 ± 1.2706), (10.474 ± 2.0546), (.3966 ± 0.15175) and (20.458 ± 509672) respectively in case group. Whereas were found in control group (283.78 ± 59.283), (9.4101±1.0148), (11.454 ± 1.6375), (0.2632 ± .05468) and (21.014 ± 6.8568) respectively . The mean of HbF in SS (20.12±14.25), AS (17.45±27.134) and not found in AA individuals. The highest level of HbS was seen in SS (75.38±15.25), AS (42.88±17.294) and not found in AA persons. The level of HbA was found to be highest in AA individual (97.34 ± 0.47), AS (34.92 ± 28.92) and not found in SS individuals.HbA2was observed in little amount in SS (3.0 ± 0.65), AS (3.19 ± 0.801) and AA (2.7 ± 0.47) individuals.