Determination of the Campylobacter Contamination of Cattle Meat in Khartoum State

Abstract

This study was conducted to determine the Campylobacter contamination of cattle meat in Khartoum State using isolation, identification and total viable count of the Campylobacter spp and sensitivity of this species to different drugs from April 2016 to August 2017. A total of 40 swab samples were collected from Al-Kadaro Slaughterhouse in Khartoum North (Bahry) and Karrari Slaughterhouse in Omdurman for total viable counts (TVCs), sensitivity test and to detect contamination. Those samples were categorized as follow : 20 swab samples From 6 cattle carcasses, which randomly selected from each slaughterhouse, Swab from the knife and another swab from worker hands, 6 swabs from the carcasses after skinning, 6 after evisceration and other 6 swabs sampled from the carcasses after two hours of chilling using sterile swabs. TVCs was 2.48 ± .037 log10 cfu/ml after evisceration and 2.32 ± .028 log10 cfu/ml after 2 hours of chilling. While the highest level of TVC occurred after skinning which is 4.44 ± .83 log10 cfu/ml, both hands workers and knives were Campylobacter free (Table 1) and the percentage of Campylobacter contamination was 55% of total samples. Sensitivity test against certain antibiotics ranged from 4.6 to 59.1% for the total samples, the highest percentage of sensitivity occurred in clostin 10 μg, while the percentage of resistance ranged from 40.9 to 95.4% in azithromycin and kanamicin 30 μg (Table 2).