Immunohistochemical Detection of E-cadherin in Urinary Bladder Tumors

Abstract

This analytical retrospective case control is hospital based study was conducted at Epn Sina hospital , and Sudan University of Science and Technology –college of medical laboratory science ,during the period from August 2016 to Mayo 2017 ,aimed to detect the expression of e-cadherin in benign and malignant bladder tumor using immunohistochemistry . 39 paraffin block samples were obtained from patients previously diagnosed as bladder tumor, 20(51%) of them were malignant and the remaining 19(49%) were benign samples. From each blocks 3µ section was cut by rotary microtome, then stained by immunohistochemical method (new indirect dextran polymer immune peroxidase technique). Data obtained was analyzed using SPSS program version 20, mean, frequency and chi square test were calculated. The age of patients ranged between 6-80, with mean age 48 years the study revealed that most patients were older than or equal 55years representing 21(53.8%)and the remaining 18(48.2) were younger than 55 years. The study found most of the patients were male26 (66.7%) with ratio of 2:1 between male to female. The study found the expression of E- cadherin in benign tumors was positive in 16(41%)cases and negative in 3 (7.7%) cases were negative while in malignant tumors 7 (17.0%) cases were negative and 13(33.3%) cases were positive, with no significant relation between E cadherin expression and type of bladder tumors(P = 0.157) . The study conclude there is no relation between E cadherin expression and type of bladder tumors.