Antimicrobial Activity of Cymbopogon proximus, Azadirachta indica and Guiera senegalenisis Plants Against Multi Drugs Resistant Bacteria Isolates From Wound Infection

Abstract

Bacteria have proved remarkably developing resistant to a known antibiotic, however, Medicinal plants have manifested notably effective for the control of bacterial infections. The aim of this study was isolated resistance bacteria from wound infection, then examine the antibacterial activity of different concentration of the ethanolic extracts of Cymbopogon proxmius, Azadirachta indica and Guiera senegalensis plants against it. One hundred samples were collected from wound infection, isolation and identification was done according to Gram stain, biochemical test and test for susceptibility to number of antibiotics. 94 showed bacterial growth, 40 (42.6%) isolates were Gram-positive bacteria (Staphylococcus aureus) and 54 sample Gram-negative bacteria (15 (27.8%) Pseudomonas aeruginosa, 13 (24.1%) Proteus spp, 12 (22.2%) Escherichia coli, 10 (18.5%) Klebsiella pneumonia, 4 (7.4%) Citrobacter freundii ). Three ethanolic extracts from Sudanese medical plants namely Azadirachta indica, Cymbopogon proximus, and Guiera senegalensisn, used in various infectious disorders, were screened for their antimicrobial properties against 50 multi-drug- resistant Gram positive and Gram negative bacteria isolated from wound infection and standers. Screening was carried out at 100 mg/ml concentration by agar disc diffusion and agar well diffusion method, the result revealed that all plants extracts were active against resistant bacteria with exception of few strains were inactive. The activity of these extracts is concentration dependent with MIC ranges from 50-6.25 mg/ml. These plant species have a promising level of activity against bacteria including strains resistant to antibiotics. The Cymbopogon Proximus whole plant ethanolic extract was more active against Gram-positive bacteria than gram-negative bacteria