Abstract:
This is an analytical retrospective case control hospital based study was
conducted in Radiation and Isotope Center Khartoum (RICK), during
the period from August 2016 to February 2017. The study aimed to
detect Survivin expression in non-Hodgkin᾿s lymphoma among
Sudanese patients using immunohistochemistry.
Forty paraffin embedded blocks from patient’s samples previously
diagnosed as non-Hodgkin᾿s lymphoma were collected. Samples
include 26 (65%) malignant tumors, (including diffuse large B.cell
lymphoma 17 (42.5%) samples, Burkhits lymphoma 2 (5.0%) samples,
anablastic large cell lymphoma in 1 (2.5%) samples, B.cell non-
Hodgkin᾿s lymphoma 2 (5%) samples, follicular lymphoma 2 (5%)
samples, small lymphocytic lymphoma 2 (5%) samples) and 14 (35%)
reactive hyperplasia benign samples. The patient’s age ranged between
22 and 65 years with mean age of 45 years, most patients were above
40 years representing 28 (70%) and the remaining 12 (30%) patients
were under 40 years.
One section of 3 micrometer thickness was cut from each paraffin
block by rotary microtome and stained by immunohistochemical
method (indirect streptoavidin-biotin immunoperoxidase technique) for
detection of Survivin. Data collected from patients files and results
obtained were analyzed using SPSS computer program.
Immunohistochemical expression of survivin was revealed positive
expression in 20/26 samples and negative expression in 6/26 samples in
malignant, while benign samples revealed positive expression in 4/14
samples and negative expression in 10/14 samples for survivin with
significant statistical association between survivin expression and
histopathology diagnosis of non-Hodgkin᾿s lymphoma (P.value =
0.006).
This study concludes that Survivin expression is associated with
malignant forms of non-Hodgkin᾿s lymphoma.
