Chemical Characterization and Biological Activity of some Flavonoids from Acacia nilotica var. tomentoza, Indigofera oblongifolia Stems and Acacia ehrenbergiana Roots

Abstract

This research was aimed to study the flavonoids of the heartwood of Acacia nilotica var. tomentosa, Indigofera oblongifolia, and the roots of acacia ehrenbergiana. Compound I was isolated from Acacia nilotica var. tomentosa. Compound II was isolated from Indigofera oblongifolia while compound III was isolated from Acacia ehrenbergiana. These compounds were purified by different chromatographic techniques and identified via spectroscopic tools;UV, and IHNMR.Depending on their spectral data , they were assigned the following partial structures: v The isolated compounds were evaluated for their antiaimicrobial potential against standard pathogenic microbes. Compound I has been screened for antimicrobial activity against six human pathogens. It showed excellent activity against Pseudomonas aeroginosa and Bacillus subtilis. It also exhibited moderate activity against Escherichia coli. Compound II showed good activity against Escherichia coli, Pseudomonas aeroginosa and Bacillus subtilis It also showed weak activity against Aspergillus niger and weak anticandidal potency. The compound also exhibited partial activity against Staphylococcus aureus. Compound III showed excellent activity against Bacillus subtilis. It also exhibited good activity against Pseudomonas aeroginosa, Aspergillus niger and good anticandidal potency. However, it showed parial activity against Escherichia coli, and Staphylococcus aureus. The isolated compound were evaluated for their antioxidant activity.The antioxidant capacity of compound I has been measured. Evaluation of the antioxidant activity was carried out by measuring the capacity of the test compound against stable DPPH radical. Compounds I,II and II exhibited significant radical scavenging capacity.