Measurement of the Prothrombin Time, Activated partial thromboplastin time and Fibrinogen concentration in healthy dogs in Sudan

Abstract

The aims of this study was to determine the mean values of prothrombin time (PT), activated partial thromboplastin time(APTT), and fibrinogen concentration(FIB) for healthy police dogs (Labrador Retriever (20) and,German Shepherd (26) ) in Sudan ,and to evaluate the effects of the breed and sex on these parameters. 46 male (21) and female (25) dogs aged between (20-83 months) were enrolled in the study. Blood samples(2ml) were taken from the cephalic vein in a tube containing 3.2% trisodium citrate.The analysis was performed using semiautomatic BCA-2000-LED-light-coagulometer-with-Double-channel and Spinreac reagents kits for human medicine. The overall mean values were 7.35±0.91second 15.12±1.32 second and 341.54±55.16 mg/dl for PT, APTT and fibrinogen concentration respectively. German Shepherd dogs showed significantly (P≤0.05) higher values than Labrador Retriever dogs for PT (7.58±1.01 vs 7.06±0.67) seconds respectively,and APTT (15.28 ±1.58 vs 14.9±0.85) seconds respectively.Males showed significantly (P≤0.05) higher values for FIB than the females (346.30±38.75 vs 337.73± 65.99) mg/dl respectively. Effect of breed should be considered in clinical interpretation of dog PT and APTT, also effect of sex should be considered in clinical interpretation of dog FIB.The determined reference values may be useful information for an increasing clinical use.