Survival of Bifidobacterium Longum BB536 in Fermented Peanut Milk Supplemented with Gum Arabic during the Storage

Abstract

The study aim to evaluate the survival of the approved probiotic strain with health benefits Bifidobacterium longum BB536 and related physicochemical changes during refrigeration storage of roasted fermented peanut based milk supplements with different types of Gum Arabic. Peanut was roasted (130°C for 20 min), soaked in water (12 h), blended (5 min) and filtered using a double layered cheese cloth to obtain the roasted peanut milk. The obtained milk was supplemented with 10% (V/V) Gums Arabic solution. After sterilization of milks it was inoculated with 3% (V/V) active probiotic strain Bifidobacterium longum BB536 and incubated under controlled conditions at 37ºC for 24h. The fermented milks were held at refrigerator temperature for two weeks. Different analyses were conducted, including: strain BB 536 survival, pH, TSS, total sugars, acidity, and moisture. The results showed significant (p<0.05) decreases in the total count of strain BB 536, pH, TSS, total sugars and moisture. However, significant (p<0.05) increases in titrable acidity and moisture were recorded during the storage. The rates of strain BB 536 reductions in first week refrigeration storage were 2.04, 2.75, 2.25 , and 2.06 log CFU /ml in fermented peanut milk, fermented peanut milk supplemented with Acacia senegal, fermented peanut milk supplemented with Acacia seyal, fermented peanut milk supplemented with gums mix (Acacia senegal + Acacia seyal), respectively. While on the second week the reductions were 1.19, 1.0, 0.95 and 1.08 log CFU/ml in fermented peanut milk, fermented peanut milk supplemented with Acacia Senegal, fermented peanut milk supplemented with Acacia seyal, fermented peanut milk supplemented with gum mix, respectively. The final viable count of strain BB536 in fermented peanut milk supplemented with gums mix after two weeks refrigeration storage exceded the minimum number required for the probiotic to exert health benefits upon consumption which was 6 log CFU /ml. Therefore, fermented peanut milk supplemented with gums mix (Acacia senegal + Acacia seyal) is a good carrier for Bifidobacterium longum strain.