The Effect of Surgery and Anaesthesia in Clinical Parameters and Plasma Glucose Concentrations with LDH Enzyme in Donkey (Equus asinus)

Abstract

This study was carried out to examine the effects of an aesthesia and surgery on clinical parameters such as heart rate, respiratory rate, rectal temperature and also the effects on plasma glucose concentrations and LDH enzyme on donkeys. Nine clinically healthy donkeys were used. The study consist of experiments trials, in the first trial (A) the animals received xylazine 2% (0.25 mg/kg) then after five minutes they received Diazepam 0.5% (0.25 mg/kg) and Propofol 1% (2mg/kg) intravenously. The second trial (B) was conducted by the injection of the above an aesthetic regime and immediately after recumbency surgical incision took place in the flank region through the skin and abdominal muscles. In both experiment A (an aesthesia) and B (surgery) heart rate, respiratory rate and rectal temperature were taken at 5, 10, 30 and 45 minutes after injection. Plasma glucose concentrations and plasma LDH enzyme were measured at 3, 6, 9, 24 and 48 hours after injection. Before administration of anaesthetic regime clinical parameters, glucose concentration and plasma LDH enzyme were measured (Controls). The sleeping time (an aesthetic phase) in experiment (A) was 18.11±5.3 minutes compared to 20.89±7.6 minutes in experiment (B). Recovery was smooth and of a good quality. In both experiments (A) and (B), heart rate significantly increased compared with the (Controls), respiratory rate significantly decreased (P<0.01) when compared to baseline values, body temperature showed significant decrease (P<0.05) at the 30, 45 minutes after injection. Plasma glucose concentrations revealed a significant different (P<0.01, P<0.05) at 3, 24 hours in both A and B.There was a significant difference in LDH after 24 hours from injection of propofol (P<0.05). It is concluded that the an aesthetic regime alone (A) or followed by surgery (B) affectedthe heart rate, respiratory rate, rectal temperature, plasma glucose concentrations and plasma LDH enzyme. The study recommended to use this regime of an aesthesia in donkey’s surgery.