Aflatoxin Content of Sesame (Sesamum indicum l) Oil in Some States of Sudan

Abstract

This investigation was carried out to determine the aflatoxin content and the physical and chemical characteristics of two types of sesame oil (Walad) (W) and (Normal sesame oil) (N), 104 samples were collected during two seasons (50 samples in June/2009, 54 samples in January/2010) from traditional mills in five states of .(Sudan (Khartoum, Kordofan, Gadarif, Blue Nile and Sennar state Two types of sesame oil in two seasons are assessed for aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and aflatoxin G2 .((AFG2), using high performance liquid chromatography (HPLC The physical testes were refractive index, viscosity, specific gravity and color, the chemical testes were acid value and peroxide value, and all 9 samples were subject to disclosure of sesame oil which has positive .result There are significant differences (P≤0.05) in physical and chemical characteristics of sesame oil in two seasons due to variation in variety of .sesame seeds, soil types and production season Aflatoxin B1 levels in sesame oil samples ranged from 0.5–9.8 μg/kg and were 0.5-1.3 μg/kg of aflatoxin B 2, which were within the .(permitted range (10 μg/kg for total aflatoxins Aflatoxin B1 (AFB1) was detected in seventy samples representing 67.31%. While aflatoxin B2 (AFB2) was detected in eight samples representing 7.69%. The highest incidence of aflatoxin B 1 contamination occurred in season II for N samples, Khartoum state had recorded the .(highest incidence (91.67% Aflatoxin B2 contamination recorded the highest incidence in season II for N sample, Khartoum state had recorded the highest incidence (12.50%). While no aflatoxin contamination with AFG1and .AFG2 was detected in any samples of sesame oil High aflatoxin contamination levels was observed in season II (long storage period) on normal sesame oil samples more than walad sesame oil samples .This results show that the aflatoxin levels in sesame oil seeds is dependent on type of seeds, storage condition and storage .period