Immunohistochemical Detection of CD20 Among SudaneseMycetoma Patients

Abstract

This is aretrospective descriptive study was conducted in Soba universityhospital during the periodfromJanuaryto December2016. The studywas aimed to detect the expression of CD20 inmycetoma. Atotal of 30 fixed paraffin blocks of patients samples previously diagnosed as tissue affected withmycetoma were collected, 7 (23.7%) samples were actinomycetomaand 23(76.3%) samples were euomycetoma. One section of 3 microns was cut from each block and stained by immunohistochemicalmethod(avidin biotin technique) for CD20 detection. The obtaineddata was analyzed using SPSS computer program version 11. 5. frequancies, percent, mean and chi square were calculated. The patients ages ranged between 12-87 years with mean age of 49years, mostof them 27(90%) were under 50 years and the remaining 3(10%)were above50years. Regardingpatient sex, the study revealed that 20(66.7%) patients were male and 10(33.3%) patientswere female. CD20 revealed positive result in 12(40%)samples, while 18(60%)were negative. 10(33. 3%) of euomycetoma cases were positivefor CD20, while 13(43.3%)were negative for CD20. In actinomycetoma two cases(6.7%)were positive for CD20, while 5(16.7%) cases were negative for CD20, with insignificant relation between CD20 expression and mycetom(p.value 0.498). The study concluded that there is no relation between CD20 expression and causative agents.