Laser Induce Fluorescence Spectra Analysis of Glucose in Human Blood Plasma

Abstract

There is an urgent need to develop technology for continuous in vivo glucose monitoring in subjects for diabetes mellitus. Problems with existing devices based on electrochemistry have encouraged alternative approaches to glucose sensing in recent years, and those based on fluorescence intensity and lifetime have special advantages, including sensitivity and the potential for non-invasive measurement when near infrared light is used. In this research twelve sample of blood with different concentrations of glucose have been employed to detect the fluorescence that induced using nitrogen laser. Plasma was separated from the twelve blood samples then it was exposed to nitrogen laser with wavelength 337.3 nm, power 0.04 mW, and periodic time is 100 msec. The fluorescence was collected using the fluorescence spectrometer. The results showed that when the concentration of glucose increased, the levels or intensity of albumin and globulin exponentially decreased. At the low concentrations of glucose less than150 Mg|Dl , the levels or intensity of protein (albumin and globulin) was very high; more than 3000 and when a concentration of glucose greater than 150, the level of protein (albumin and globulin) decreases .until reach low levels or intensity 200 At high concentrations of glucose 545 Mg / DL. Decreasing the level of protein (albumin and globulin) indicated to attribute to a decrease in the body's insulin.