Clinical Trial of a locally Produced Inactivated Newcastle Disease Vaccine

Abstract

This study was conducted in order to investigate the safety and efficacy of a newly product,it describe the production of Newcastle disease vaccine for first time in Sudan from the (I-2) Australian strain. A vaccine batch was produced using the freeze dried working seed which was propagated by inoculated of 10 days oldembryonatedchicken eggs,chorioallantoic fluid was harvested and tested for absence of contamination. The virus was inactivated using 0.5% highly purified formalin,then the vaccine was formulated as water in oil emulsion. The vaccine safety and efficacy were tested, using 120 one day old broiler chicks, which were randomly divided into 3 groups, each containing 40 chicks. Group 1 was a control, group 2(safety group) was inoculated with 0.4 ml of vaccine S/C, group 3 (efficacy group) was inoculated with 0.2 ml of vaccine. Sera were collected from the control and efficacy groups before vaccination for evolution maternal immunity on days 14, 21, and 30 post vaccination for evolution ofseroconversion. Haemagglutination Inhibition revealed a significant difference in antibodies, the p < 0.05 between efficacy and control group. The safety group was observed during optimal time span, where no clinical signs or mortality was seen. These results confirmed the safety and efficacy of the Newcastle inactivated vaccine under laboratory condition. The vaccine needs to the tested under field conditions.