Measurement of Plasma Von Willebrand Factor Antigen Level among pregnant Sudanese Ladies in Third Trimester

Abstract

Von Willebrand factor (vWF) is a large sialoglycoprotein , that circulates in normal plasma as a series of heterogeneous multimers and plays a critical role in primary hemostasis by mediating platelet adhesion to exposed collagen at sites of vascular injury. Von Willebrand factor is blood clotting protein and one of several components that work together and in sequence to stop bleeding by forming a blood clot. High molecular weight multimers of Von Willebrand factor demonstrate enhanced binding affinities or both collagen and platelets and are therefor more efficient in mediating platelet recruitment.This study aims to evaluate the vWF level among Sudanese pregnant women’s. A total of 40 sample were collected (from age 18 to 41and different trimester) from pregnant women .sThe blood was collected from each subject by clean venous puncture into sample container; containing 0.5 ml of 3.8 Tris odium citrate for the estimation of vWF : Ag and gently mixed and then separated immediately at 4500 rpm for 15 min after which platelet poor plasma (ppp) was collected in plain container and stored at -20 ˚C until analysis and control also take 40 sample from normal ladies non pregnant.ThevWFlevel was measured using immune assay ELISA .Data were analyzed by statistical package for social science (SPSS). The result showed that there is significant association between pregnancy and plasma vWF level (p-value=0.039)but the result show no significant association between vwf and age (P-value=0.658) and Wight (P-value=0723)Von Willebrand factor testing measures the quantity and function of Von Willebrand factor. The study concluded that , the vWF is increase in thirdtrimester pregnant women which can leads to many problem and disorder.