Abstract:
The study was aimed to isolate and to identify three types of bacteria at six different operational processes in Traditional poultry slaughtering process in Khartoum state. 90 swab samples were collected from carcasses of broiler chicken at six processing stages (Scalding, defeathering, evisceration, after washing, after chilling, hands of Employees.) during slaughtering process. Traditional methods were used to determine the isolation and identification of three bacteria (Salmonella spp., Escherichia coli and Staphylococcus aureus). The results revealed that, the highest contamination level was at Evisceration which showed high total bacterial count (mean (log10CFU/ml) 7.43 ±0.16)) and the numbers of bacteria isolated as follows 12(6.9%) samples were positive for Salmonella spp., 10(5.7%) samples were positive for Escherichia coli, and 8(4.6%) samples were positive for Staphylococcus aureus, the low level contamination after Chilling which showed (mean(log10CFU/ml) 7.30 ±0.14) and the numbers of bacteria isolated were 14(7.3%) samples were positive for Salmonella spp., 7(4%) samples were positive for Escherichia coli and 10(5.7%) samples were positive for Staphylococcus aureus. The results at scalding stage showed total bacterial count (mean (log10CFU/ml) 7.28±0.11) 14(8%) and the numbers of bacteria isolated as follows samples were positive for Salmonella spp.,6(3.4%) for Escherichia coli, and 9(5.1%) for Staphylococcus aureus The results at defeathering stage showed total bacterial count (mean (log10CFU/ml) 7.43±0.08) and the numbers of bacteria isolated as follows 12(6.9%) samples were positive for Salmonella spp., 8(4.6%) for Escherichia coli, and 6(3.4%) for Staphylococcus aureus. The results after washing stage showed total bacterial count (mean (log10CFU/ml) 7.36±0.11) and the numbers of bacteria isolated as follows 12(6.9%) samples were positive for Salmonella spp., 3(1.07%) for Escherichia coli, and 10 (5.7%) for Staphylococcus aureus. The results at employees stage showed total bacterial count (mean (log10CFU/ml) 7.37±0.16) and the numbers of bacteria isolated as follows 12 (6.9%) samples were positive for Salmonella spp., 10 (5.7%) for Escherichia coli, and12 (6.9%) for Staphylococcus aureus. The statistical analysis of results revealed that, there was significant difference at P-Value (P≤ 0.01) in all Different Operational Points in traditional slaughtering process in Khartoum state.
