Detection of Some Virulence Genes of Pseudomonas aeruginosa Isolated from Different Clinical Specimens by Multiplex PCR, Khartoum, Sudan

Abstract

Pseudomonas aeruginosa possesses a variety of virulence factors that may contribute to its pathogenicity,it also frequently resistant to many commonly used antibiotics.The aim of this study was to detect P. aeruginosa oprI, oprL,toxA, exo S, nan1 and LasBvirulence genes by Multiplex Polymerase Chain Reaction (PCR). A total of 81isolates of P.aeruginosarecovered from urine, wound , blood, otitis and respiratory tract during the period from 15th March to 18thof June were collected and reidentified by conventional and molecular methods. positive isolates for oprI and oprL genes were further tested for the presence of other virulence genes and antibiotic susceptibility testing. The antibiotic susceptibility test found that all 70 (100%) isolates were resistant to amoxicillin and amoxyclav, and mostly sensitive to imipenem 64(91.4%), gentamicin 56 (80%), ciprofloxacin 50 (71.4%), and ceftazidime 33(47.1%). Multiplex PCR was used for detection of virulence genes of P.aeruginosa. Only 70 (86%) isolates were positive for oprI and oprL genes, from these 70 isolates toxA gene detected in 60 (85.7%), lasB gene in 64 (91.4%), exoS gene in 37(52.9%) and nan1 gene in 23(32.9%). There was significant correlation between presence of virulence genes and susceptibility to antibiotics.