Serological Detection of Hepatitis B virus in healthy individuals in Marrwy and Karima - Northen State

Abstract

Hepatitis B infection is one of the world's major infectious diseases . This was across-sectional study was conducted during March to June 2017, to determine the sero-detection of Hepatitis B virus and to determine the possible association between Hepatitis B virus and some risk factors among health population in Northern State. structured questionnaire was used to collect both demographic and clinical data. Blood samples were obtained from eligible participants, sera were separated by centrifugation for detecting Hepatitis B surface antigen (HBsAg). A local antiseptic (70% ethanol) was used to clean the skin. Venous blood (3mLs) were taken from each participant by standard procedures and were put in plain containers to clot then centrifuged at 1500 rpm for 5 minutes and serum was obtained and kept at -20˚C until used. The serum samples were examined by Enzyme Linked Immunosorbent Assay (ELISA) to detect HBsAg .Six out of 92(6.5%) samples were found HBsAg positive by ELISA .The study indicated insignificant (P>0.05) The distribution of positive cases as follows Gender: 5 (5.4%) of cases were males and 1(1.1%) was female, Age: The highest number (5.5%) was distributed at age group (31-65) years and there was 1 infection between age group (15-30). Hepatitis B virus were detected in six of each of the following group single , non married , non vaccinated,had no blood transfusion ,and there who have no history of surgical operation. Further studies are required with large number of participants to confirm these results by using molecular techniques .