Abstract:
Streptococcus agalactiae (S.agalactiae) is one of the most
important etiologic agent of several infection,cause abortion, and
a present a major public health problem. This study aimed to
detect the frequence of S. agalactiae among pregnant women with
previous miscarriage by using PCR.Fifty samples of vaginal and
high vaginal swabfrom pregnant women , were collected from
different hospitals in Khartoum,25 from each vagina and high
vagina.
Patients’s data was collected by structured interview questionnaire
and analyzed by statistical package for social sciences (SPSS),
version 11.5.
DNA was extracted by boiling method and alcohol was used to
concentrate the nucleic acid . PCRtechnique was used to amplify
the DNA of Group B Streptococci (GBS), the result showed 4 (8%)
of each vaginal and high vaginal swabs were positive GBS.
Thirty four (68%) from subjects were normal delivered, 8 (16%) were
caesarean section delivery, and 8 (16%) were no previous delivery,
Frequency of GBS according to age grouping (19-26), (27-34) and
(35-42) were positive ± negative ( 0 ± 11 ; 5 ± 20 ; 3 ± 11)
respectively . Mean and standard deviation of agepregnant women,
Mean± SD (30.8±5.4) respectively, frequency of Streptococcus
agalacticae among pregnant women with previous miscarriage,
positive (16%) and (84%) negative, the mean of age according to
freqeunce of Streptococcus agalacticae positive (33%) and (30%)
negative.
Finally found the frequency of Streptococcus agalacticae in pregnant
women with previous miscarriage according to type of delivery
5(10%) normal delivery, 1(2%) caesarean section delivery and 2(4%)
no previous delivery.
