Molecular Detection of Human Papillomavirus Type 16 and 18 DNA in Cervical Cancer Tissue Biopsies from Sudanese Women Attended Military Hospital During 2015-2016

Abstract

The aim of this study was detection of Human Papillomavirus type 16 and 18 DNA in cervical cancer tissue biopsies from Sudanese women attended to Military Hospital by multiplex PCR. A total of 45 cervical carcinoma biopsies (paraffin embedded and previously diagnosed as cervical cancer carcinoma by histological examination) were collected from Military Hospital. The specimens were treated with Xylene and different concentrations of ethanol for the removal of paraffin wax. Then the DNA was extracted by Salting out method (6 M NaCl), Finally Multiplex PCR was done for the detection of E6 gene of HPV 16, and E7 gene of HPV18. The study population were 45 females which were previously diagnosed with cervical cancer and their ages ranged from 40-70 years with the mean age of 55 years. Out of the total 45; 17(38%) showed positive results for HPV 16 and 18 genes, while 28(62%) were negative. The results also showed that HPV16 was detected in 12/17(71%)of the positives, and HPV 18 was detected in 5/17(29 %), HPV 16 and 18 as a co-infection were not detected. According to the residence of patients HPV16 and 18 were found mostly in Omdurman8/17(47%), followed by Khartoum 4/17(24%), Bahri 2/17(11%) and Out of Khartoum State 3/17(18%), and according to the age of the patients HPV 16 and 18 were found mostly in the age group 61-70 years 8/17 (47%), followed by age group 51-60 years 6/17(35%) and age group 40-50 years 3/17 (18%). The study concluded that there was no significant association (P-value 0.649) between the HPV 16 and 18 with the residence of the patients, and also there was no significant association (P-value 0.088) between HPV 16 and 18 with age groups.