Aerobic Bacteria Isolated from Infertile Dairy Cows (Repeat Breeders) in Gezira State, Sudan

Abstract

The aim of this research was to study the role of the aerobic bacteria persisted in vaginas of repeat breeder dairy cows in Gezira State-Sudan. One hundred and ninety two samples of sera, vaginal swabs and cervico-vaginal washes were collected from local and cross breeds of repeat breeder cows during the period May 2012 to May 2013. Serum samples were tested for brucella antibodies by Rose Bengal Test (RBT), while vaginal swabs and cervico-vaginal washes were used for bacterial isolations. The vaginal swabs were cultured on both serum dextrose agar and blood agar media for the isolation of Brucella spp. and other related bacteria respectively while cervico-vaginal washes were cultured on Skirrow s selective medium for Campylobacter. The isolated bacteria (except Bacillus spp.) were identified using the VITEK® 2 Compact system while Bacillus spp. were identified according to Barrow and Feltham (2003) method. 25 %( 11) of the tested sera were found positive for brucella antibodies; however, on cultures only 2 brucella isolates were recovered. On Blood Agar medium, 89.1% (57) of the vaginal swab samples showed bacterial growth and 87 bacterial isolates were recovered from these cultures. The predominant bacteria were Staphylococcus spp. (24.1%) followed by Kocuria spp. (18.4%) and both of Granulicatella spp. and Bacillus spp. (5.7%). Some bacterial spp. were isolated for the first time in Sudan and many of them such as: Streptococcus pneumoniae, Streptococcus pluranimalium, Gardenella vaginalis, Providencia rettgeri, Streptococcus dyssagalactiae subspp. equisimilis, Aeromonas hydrophila and Burkholderia cepacia act as significant pathogens for bovine and human .Although the prevalence of some bacteria Myroides spp., Cronobacter sakazakii group, and Gemella bergeri in animals was not globally proven yet, they were found in this study inhabiting the vaginas of repeat breeder cows which is an unexpected findings. Campylobacter fetus bacterium was not isolated from the cervico-vaginal washes