Effects of Lycopene on Paraoxonase and Adipokines Parameters in Streptozotocin - Induced Diabetic Rabbits

Abstract

Background: Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycaemia resulting from defects in insulin secretion, insulin action, or both, and occurs in almost all populations of the world a variable prevalence. Objectives: The current study assessed the impact of lycopene on adipokines and paraoxonase (PON) in stereptozotocin-induced diabetic rabbits. Materials and Methods: A total of one hundred of rabbits (Albino Rabbits) of both sexes weighing between 1.5-2.1 kg were completely randomized in four groups (G) comprising 25 rabbits each. Diabetes was induced by a single intraperitoneal injection of streptozotocin (35 mg/kg body weight) feeding on a high fat regimen. Rabbits in G 3,4 which were administered 10 and 20 mg/kg respectively of lycopene for 4 weeks. The serum levels of leptin, adiponectin, ghrelin, resistin, and tumour necrosis factor – α (TNF-α) were measured by enzyme-linked immunosorbent assay (ELISA), while serum PON was measured by spectrophotometric method. SPSS computer software was used for data analysis. Results:Results showed that lycopene administration at both doses significantly reduced the leptin, resistin, TNF-α, and glucose levels compared to diabetic control groupG2. Consistent significant increases (p < 0.05) were noticed in the level of paraoxonase, adiponectin, and ghrelin in diabetic rabbits dealt with lycopene in comparison with diabetic rabbits of control group G2. Conclusions: The high leptin, resistin, and TNF-α levels and oxidative damage whereas low linked with diabetes, were improved with the administration of lycopene.