Molecular Detection of Plasmids Mediated Colistin Resistance (MCR-1) Gene in Enterobacteriaceae from Clinical Specimens in Khartoum State

Abstract

The aim of this study was to detect the presence of MCR-1 gene in clinical isolates of Enterobacteriaceae in Khartoum State, Sudan,at the period between February to July, 2016. Fifty clinical samples were collected and cultured on MacConkey agar, the grown bacteria was identified by their growth criteria, Grams stain and biochemical tests. Antimicrobial susceptibility was done for them by using the following antibiotics; ciprofloxacin, gentamicin, co-trimoxazole, cefotaxime, cefuroxime and cefixime. DNA was extracted by using bacterial genomic extraction Kits. PCR was done by using MCR-1 gene specific primer. The most commonly isolated organism was E. coli 38 (76%), followed by Proteus spp. 8 (16%), and K. pneumoniae 4 (8%). Males (60%) weremore infected than females (40%), and elderly patients 61-90 years (42%) are found to be more susceptible to infection. We also reported highly resistant rate to ciprofloxacin (66%), gentamicin (68%) and cefuroxime (96%). MCR-1 gene was detected in 7 (14%) isolates, mostly in E. coli. We reported for the first time in Sudan the presence of MCR-1 gene in clinical isolates, and the prevalence of this gene is higher when compared to other countries.