Measurement of Plasma Von Willebrand Factor Antigen Level among Sudanese Patients with Type 2 Diabetes Mellitus

Abstract

This is observational analytical case control study was carried out to estimate plasmavwf (Ag) level in type 2 diabetic patient.It was conducted in Alribat national hospital in Khartoum state during the period from January 2017 to August 2017.The study included 80 samples;40 samples from type 2 diabetic patient (cases) and 40 samples from healthy individual (control) ;2.5 ml of venous blood was collected in avacotainer tube containing 0.25ml of 0.38% tri sodium citrate and then separated by centerfugation and plasma was collected to measure von willebrand factor using Enzyme linkageimmunosorbent Assay. The results was analyzed by statistical package for social science computer program (spss) version 16.The data obtained through questionnaire. The results showed that there is statisticaly significant difference in the mean of vwf(Ag) level between cases and control p.value=0.002 and the mean of vwf (Ag) among cases =0.76 IU/ml and the mean of vwf (Ag) level among control =0.66 IU/ml. In the present study I observed that there is strong positive correlation between vwf (Ag)level and the age of the diabetic patients p.value= 0.01and pearson correlation=0.89 and also show positive correlation between vwf (Ag) and the duration of the diabetes pvalue=0.03and pearson correlation=0.22; in addition there is appositive correlation vwf(Ag) level and fasting blood glucose p.value=0.04 and pearson correlation=0.15. Finaly this study showed there is no significant difference in the mean of vwf (Ag) level between male and femalep.value=0.064.