Association of Interleukin-10 (1082G/A) (rs1800896) Polymorphism in Acute Myeloid Leukemia Patients in Sudanese Population

Abstract

This is prospective case control study conducted in Radio isotope center Khartoum Sudan to study a single nucleotide polymorphism (SNP) in IL-10 -1082G/A (rs1800896) in Sudanese acute myeloid leukemia patients in Khartoum state in the period from January to May 2017. Thirty samples were collected from newly diagnosed patients of acute myeloid leukemia and 30 samples were collected from normal individuals as control group, all samples were collected in Ethylene di-amine tetra acetic acid (EDTA) container, then Deoxyribonucleic Acid (DNA) was extracted by using salting out method. And polymorphism analysis for IL-10(-1082G/A) (rs1800896) was determined by using allele specific-PCR. The results were analyzed by statistical package for social sciences (SPSS) computer program. The results showed that (36.7%) of cases have homogenous IL-10 (1082G/A) genotype, (43.3%) have heterogeneous (IL-10 -1082G/A) genotype, (20.0%) have normal IL-10(-1082G/A) genotype. The association between IL-10 (-1082G/A) polymorphism and AML is statistically significant, heterogeneous genotype of IL-10(-1082G/A) polymorphism is risk factor of AML. The study concluded that there is no association between IL-10(-1082G/A) polymorphism and AML sub-type, gender, age group, mean of hematological parameter (TWBs, Blast%, Hb and platelet count).