The effect of Estradiol Benzoate Injection on some Blood Constituents and Prostate Gland Tissue for Intact and Castrated Male Rabbits

Abstract

This study was conducted to investigate the effect of injection of Estradiol Benzoate to intact and castrated male rabbits on some blood constituents and prostate tissue. A total of Ninety mature male rabbits (54 intact and 36 castrated) were used in this study.The rabbits were randomly divided into three groups and each group was further divided into four sub-groups. The rabbits of each groups were injected estradiol benzoate dosed at 0, 40, 80 and 120 μgm/rabbit, through intramuscular (IM) route, on each alternative day over a period of 30 days, whereas group three (18 intact rabbits) were offered an oral dosage of 1mg from prolactin inhibitor (Bromocriptine Mesilate)/ rabbit in addition to estradiol. Serum LH, FSH, and Testosterone levels were performed by immunoenzymometric assay , total-protein, Albumin, glucose , urea , creatinine, lipids profiles, AST, ALT and ALP enzymes, were measured in serum samples. Automate chemistry analyser (MINDARY) and reagent Biosystem® Spain made were used for analysis . Prostate tissue samples were taken from each sub-groups, tissue processing method was made by automatic tissue processor and then embedded in paraffin wax. Sections of 5μ thick were cut by a rotary microtome, stained in Mayer’s haematoxylin and Eosin H&E (Bancrof et al., 1996). All sections were examined under the light microscope (Olympus) to describe the microscopic changes and imaged by using digital camera. The statistical analyses were done using the SPSS statistical program, version 20 for Windows (IBM SPSS Statistics 20 IL, USA),and the results were expressed in form of mean ± standard deviation. The results showed that, the mean serum levels of LH and FSH were not affected by injection of estradiol benzoate in all intact and castrated rabbits sub-groups (P>0.05), while the mean testosterone concentration levels were showed insignificant increase in both intact and castrated male rabbits, except the intact male rabbit sub-group that received estradiol benzoate at 120 μg/rabbit (P<0.05). However, the concentration levels of total protein ,glucose albumin and calcium were not affected by the injection of different doses of estradiol benzoate, Moreover, the study revealed that, the injection of estradiol benzoate at a dose of 40μgand 120 μgIM to intact male rabbits induced a significant (P<0.05) decrease in the creatinine level and increase in urea concentration in castrated rabbits. However, the result of the present study showed that, the injection of estradiol benzoate at doses of 40, 80 and 120 μgm/ rabbit intramuscularly were not induced any significant (P>0.05) changes on lipids profile of intact and castrated male rabbits, while HDL was significantly (P<0.05) increased at a dose 40 μg in intact male rabbits. However, liver enzymes AST, ALT and ALP did not affected (P<0.05) by injection of estradiol benzoate to intact rabbits, whereas, AST and ALP were significantly (P<0.05) decreased in castrated male rabbits as compare to the intact male rabbits. The correlation between estradiol benzoate doses and blood constituents had showed that, a significant positive correlation in ALT ,testosterone and significant negative correlations in glucose , triglyceride , LDL and LH in intact male rabbits, whereas, a significant positive correlation in glucose , urea , and a significant negative correlations in creatinine , triglyceride , AST ,and ALP And in castrated male rabbits were detected. Furthermore, the effects of estradiol benzoate in prostate tissues were ranged from hyperplasia with dysplasia or dysplasia only in intact male rabbits; hyperplasia was represented by papillary projection in castrated male rabbits,however, administration of estradiol benzoate to intact male rabbits in concomitance with prolactin inhibitor (Bromocrepteinmysaylate) induced mild hyperplasia in intact male rabbits. It is concluded that injection of estradiol benzoate to male rabbits induced many effects represented inLH , FSH, testosterone , total-protein, Albumin, glucose , urea , creatinine , lipids profiles , liver enzymes, there is however, strong evidence shows that excessive or untimely exposure to estrogens can facilitate development of prostatic changes, disorders and even malignancies