Usage of laser emission spectroscopy to produce singlet oxygen from Methylene blue, Eosin yellow and Fluorscin

Abstract

In this work the emission of singlet molecular oxygen at room temperature was determined by using spectroscopic method. The dyes were preparedfrom (Methylene blue, Eosin yellow and Fluorscin dissolving in methanol ethanol).Then UV visible spectrometer was used to record the absorption spectra for the solventsand the dye samples.Then the samples were irradiated by different light sources to firstly (LED (365 nm with output power 1200 mW),secondly by the green diode laser (532 nm) with output power 100m W. Thirdly the red diode laser (671 nm). USB 2000 spectrometer was used to record the emission spectra of the samples with different times start from 30sec to 120 sec. The result showed when Methylene bluedissolved in methanol and ethanol after irradiated by red diode laser (671 nm) have efficiency to produce singlet oxygen where the peak at 703 nm and Eosin yellow dissolved in methanol and ethanol after irradiated by LED (365 nm) also haveefficiency to produce singlet oxygen where the peak at 577 nm. Where singlet oxygen formation can be used in photodynamic therapy to treatment same skin diseases and destroy cancer tumor.