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Association between Angiotensin Converting Enzyme Gene Insertion/Deletion Polymorphism and Cardiovascular Diseases among Diabetic Patients

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dc.contributor.author salama, Mona Mohammed Suleiman
dc.date.accessioned 2017-07-26T09:59:57Z
dc.date.available 2017-07-26T09:59:57Z
dc.date.issued 2017-06-01
dc.identifier.citation salama, Mona Mohammed Suleiman.Association between Angiotensin Converting Enzyme Gene Insertion/Deletion Polymorphism and Cardiovascular Diseases among Diabetic Patients\Mona Mohammed Suleiman salama;Elshazali Widaa Ali.-Khartoum:Sudan University of Science & Technology,Medical Laboratory Science,2017.-54p.:ill.;28cm.-M.Sc. en_US
dc.identifier.uri http://repository.sustech.edu/handle/123456789/18416
dc.description Thesis en_US
dc.description.abstract Cardiovascular diseases(CVDs) are the most prevalent cause of mortality and morbidity among people with diabetes mellitus. On the other hand, diabetes mellitus deserves to be designated a major risk factor for cardiovascular diseases. The insertion/deletion (I/D) polymorphism of the angiotensin-converting enzyme (ACE) gene is considered to contribute to an interpersonal variability in serum ACE levels and thereby providing a plausible basis for increased susceptibility to thrombotic events. This study aimed to investigate the association between ACE gene I/D polymorphism and risk of cardiovascular diseases in diabetic patients. A total of 100 subjects were recruited for this case control study, 50 diabetic patients with CVDs and 50 healthy volunteers as a control group. Blood samples were collected from all participants in EDTA anticoagulant container, genomic DNA was extracted by salting out method, and the ACE I/D polymorphism was analysed using polymerase chain reaction (PCR). Amplified fragments separated on 2% agarose gel stained with ethidium promide and demonstrated by gel documentation system. Patients’ data was collected by structured interview questionnaire and analysed by statistical package for social sciences (SPSS), version 19. The DD genotype of ACE I/D polymorphism was more frequent in both diabetic patients with CVDs and control group(72%,74%( respectively than ID genotype(28%,26%) respectively, while the II genotype was absent in both groups. ACE I/D polymorphism was found to have has no effect on age of incidence of CVDs in diabetic patients(Pvalue 0.886). There was no interaction observed between ACE genotypes and other known CVDs risk factors in diabetic patients The results showed no statistically significant difference in mean duration of DM when compared in the two ACE genotypes, Mean± SD (12.28±8.410, 12.21±8.894 for DD,ID) respectively, (pvalue 0. 981). There was no statistically significant association between ACE I/D polymorphism and risk of cardiovascular diseases in Sudanese diabetic patients. en_US
dc.description.sponsorship Sudan University for Sciences and Technology en_US
dc.language.iso en en_US
dc.publisher Sudan University of Science & Technology en_US
dc.subject angiotensin converting enzyme en_US
dc.subject Cardiovascular Diseases en_US
dc.subject Diabetic Patients en_US
dc.title Association between Angiotensin Converting Enzyme Gene Insertion/Deletion Polymorphism and Cardiovascular Diseases among Diabetic Patients en_US
dc.title.alternative العلاقة بين تعدد الشكل الجيني إضافة / حذف للإنزيم المحول للأنجيوتينسين وأمراض القلب وسط مرضي السكري en_US
dc.type Thesis en_US
dc.contributor.Supervisor Supervisor,- Elshazali Widaa Ali


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