Immunohistochemical Detection of Alfa1-antitrepsin among Sudanese Patients with Colorectal Tumors

Abstract

This is a hospital based case control study conducted in national public health laboratory during the period fromDecember 2016 to April 2017, the study aimed to detect the expression of Alfa1-antitrpsin in the diagnosis of colorectal tumors. A total of 40 formalin fixed paraffin blocks previously diagnosed as colorectal tumors, 20(50.0%) samples were benign colorectal and 20(50.0%) samples were malignant colorectal tumors were selected for this study. One section of three microns was cut from each block and stained by immunohistochemical method (avidin biotin technique) for Alfa1-antitrpsin detection. The data obtained were analyzed using SPSS computer program version 20. The patient ages ranged between 26-80 years with mean age of 53 years,most of them 22(55.0%) were above 50 years and the remaining 18(45.0%) were less than 50 years. Regarding gender patient sex revealed that 12(30.0%) patients were female and 28(70.0%) patients were male. Alfa1-antitrepsin was positive in 21(52.0%) samples, while 19(47.0) samples were negative. From positive result 11(27.5%) benign colorectal samples while 10(25.0%) malignant colorectal samples with insignificant correlation between Alfa1-antitrepsin and colorectal tumors (P.value 0.752). The study concluded that there is no relation between expression ofAlfa1-antitrepsinand colorectal tumors.