Immunohistochemical Detection of Cyclin D1 in OvarianCancers AmongSudanese Women

Abstract

This an analytical retrospective case control hospital based study was conducted in Radiation and Isotope Center Khartoum (RICK), and national public health laboratory during the period fromAugust 2016 to April 2017. The study aimed to detect cyclin D1 expression in ovarian tumors using immunohistochemistry. Forty paraffin embedded blocks previously diagnosed as ovarian tumors were collected. Samples include 30(75%) malignant tumors (including serous carcinoma in 14 (35%) samples,mucinous carcinoma in 3 (7.5%) samples,endometrioid carcinoma in 5 (12.5%) samples, clear cell carcinoma in 2 (5%) samples, adenocarcinoma in 4 (10%) samples, germ cell carcinoma in 2 (5%) samples) and 10 (25%) benign samples. The patientʼs age ranged between 23 and 70 years with mean age of 46 years, most patients were more than 40 years representing 23 (57.5%) and the remaining 17 (42.5%) patients were less than 40 years. One section of 3 micrometer thickness was cut from each paraffin block by rotary microtome and stained by immunohistochemical method (indirect streptoavidin-biotin immunoperoxidase technique) for detection of cyclin D1. Data collected from patients files and results obtained were analyzed using SPSS computer program. Immunohistochemical expression of cyclin D1 was revealed positive exepression in 18/30 samples and negative result in 12/30 samples in malignant tumors, while all benign tumors gave negative result for cyclin D1 (0/10), with significant statistical association between cyclin D1 expression and histopathological diagnosis of ovarian tumor (P. value = 0.001). This study concludes that cyclin D1 expression is associated with malignant forms of ovarian tumors.