Preparation and characterization of physicochemical and Functional Properties of Potassium Glucuronate from Acacia seyal var. seyal Gum

Abstract

Acacia seyal var. seyal gum from Al Gadaref was used in this study. Physical and chemical properties such as moisture, ash, pH value, intrinsic viscosity, Molecular weight, specific rotation, nitrogen and protein, acid equivalent weight, total glucuronic acid, and cationic composition were determined for Aseyal gum. The values of moisture and ash found to be 11.09%, 2.77 % respectively, while the specific rotation value of the sample was +56.59˚. Intrinsic viscosity of sample was 14.7ml/g, while the calculated Molecular weight value from Mark-Houwink equation was 7. 5x105. The pH value was 4.4. while nitrogen and protein content 0.09%. ,0.528 %. The values of acid equivalent weight and percentage of total glucuronic acid were found to be 1587 and 11.5 % respectively. Results indicate that the calcium is major element present in Acacia seyal gum(2028.809ppm) when was compared with potassium(554.685), sodium(25.147) and magnesium(1120). Glucuronic acid was prepared from Acacia seyal gum by cation exchange chromatography. Potassium glucuronate was prepared by titrating glucuronic acid with potassium hydroxide. Glucuronic acid and potassium glucuronate gave pH values of 2.9,7.8 respectively. The values of moisture were 6.2%, 7.1% respectively, while the viscosities were 10.3, 12.39ml/g. The values of ash were found to be 0.38, 5.6% respectively. The specific rotation value of glucuronic acid and potassium glucuronate were +59.934˚, +55.00˚ respectively. In addition the stability of Acacia seyal, glucuronic acid and potassium glucuronate emulsions with regard to type of oil(sunflower and groundnut) were also investigated. Results indicate that emulsion stability is significantly affected by type of emulsifier and the type of oil used.