Efficacy of Terminalia Brownii Bark Extract in Treating Induced Staphylococcus aureus Mastitis in Nubian Goats

Abstract

Mastitis was experimentally induced in 3 groups of lactating goats each of 5 goats; by inoculation of pathogenic Staphylococcus aureus into each teat canal at doses of 0.1ml of an over night broth culture containing 98-109 viable bacterial count per ml, 1x10-3 and 1x10-5 dilution of the same culture into the 3 groups respectively. Methanolic extract of Terminalia brownii bark, prepared at a concentration of 25 mg/ml of normal saline was infused at doses of 1.5ml daily for 12 days into each teat canal of the infected udders. Leucocyte count in the milk of goats was used to assess the in vivo efficacy of the bark extract in treating induced Staphylococcus aureus mastitis. Leucocytes were counted every 3 days during the 12 days of therapy. On the 13th day, milk specimens were collected aseptically from each teat of the infected udders and cultured on blood agar medium for detecting the presence of Staphylococcus aureus colonies. The extract of bark treated mastitis in (2/5) 40% of the goats of group (2). Goats of group 3 and 4 mastitis were treated in 60% (3/5) each group. That was apparent by the drop of leucocyte counts in the milk to normal levels and the absence of Staph. aureus colonies on cultures of milk specimens. The data presented in this study shows that T. brownii extract of the bark can treat induced Staph. aureus mastitis in goats