Immunohistochemical Detection of HPV Ag in Oesophageal Tumours

Abstract

This study aimed to detect the expression of HPV Ag in oesophageal tumours using immunohistochemical method. Sixty formalin fixed paraffin blocks (FFPB) previously diagnosed as oesophageal tumours (50 of them were malignant oesophageal tumours and 10 were benign oesophageal tumours) were used in this study. Blocks were cut and stained by immunohistochemical method (New indirect method) for detection of HPV Ag. The data obtained were analyzed using SPSS program. The age of patients ranged between 30 and 85 years with mean age of 60 years. The study subjects included 33 (55%) males and 27 (45%) females. Out of fifty malignant samples, 41 samples were squamous cell carcinoma and 9 samples were adenocarcinoma. The immunohistochemical expression of HPV Ag was detected in 12 (20%) samples and negative in 48 (80%) samples. 10/12 positive samples were malignant and the remaining 2/12 were benign, with no statistical association between HPV infection and malignant tumours of the oesophagus (P =0.646). The degree of histological differentiation revealed 39 (78%) samples were moderately differentiated tumours, 7 (14%) samples were poorly differentiated tumours and 4(8%) samples were well differentiated tumours with insignificant statistical association with the HPV infection (P>0.05). This study concludes that there is no association between HPV infection and malignant tumours of the oesophagus