Growth of Bifidobacterium pseudocatenulatum G4 and changes in organic acid profile in peanut milk and skim milk supplemented with fructooligosaccharides

Abstract

Bifidobacterium pseudocatenulatum G4 was evaluated for its potential use in peanut milk (PM) based product. The study examined the growth and subsequent organic acids production compared with that on reconstituted skim milk (SM). The initial pH was relatively set at 6.5. Inoculums of 0.67% were monitored and samples were incubated anaerobically for a period of 27 h under control conditions using 1L volume bioreactor. The maximum growth was attained at incubation period of 24h. The growth was inspired by means of fructooligosaccharides (FOS) supplementation. High growths of 8.35 log CFU/ ml PM + FOS and 8.39 log CFU/ml SM + FOS were recorded as compared with 8.35 log CFU/ ml TPY (Trypticase Phytone Yeast Extract) media. During incubations, the pH reductions were explained by increases in lactic, acetic, probionic and butyric acids. Profiles of probionic and butyric acids in fermented PM did not significantly varied from accepted levels as demonstrated in consumable fermented SM. The maximum growth obtained in fermented samples was high, exceeding (> 7 log CFU/ml) the recommended level of ten million viable cells per ml product. Therefore, PM medium could be developed beside SM as a potential carrier for strain G4.