Comparison between Immunochromatographic Test and Enzyme Linked Immunosorbent Assay for diagnosis of Helicobacter pylori infection

Abstract

The aim of this immunochromatography study was (ICT), to versus compare Enzyme between linked immunosorbent assay (ELISA) for detection of IgG antibodies of H. pylori in patients plasma. The gold standard for the diagnosis of Helicobacter pylori infection requires an endoscopic biopsy of gastric mucosa for histological examination, urease test and culture. Noninvasive serological tests are useful as a screening test for H. pylori infection. This study was carried out during period between June to October 2012, in Khartoum state, and the plasma samples were collected from three different Hospitals (Khartoum Hospital, Albaraha Hospital and Yastabshroon Hospital). One hundred samples were collected,53 males (53%) and 47 were females (47%). All samples were subjected to ICT and ELISA, 72 were positive (38 males and 34 females), and 28 were negative by ICT (15 males and 13 females), while by ELISA; 69 were positive (38 males and 31 females), and 31 negative (15 males and 16 females). This study showed that Immunochromatography test ( ICT) is useful in the diagnosis of H.pylori infection.