Immunohistochemical Detection of Epstein Barr Viruse among Lymphoma Sudanese Patients

Abstract

This is retrospective analytical case control study conducted at Radiation Isotope Center Khartoum (RICK), during the period from April 2014 and August 2015. The study was aimed to detect the presence of Epstein Barr virus in lymphoma using immunohistochemical method. Sixty paraffin embedded blocks previously diagnosed as lymph node lesions were collected. Samples include 50(83.3%),( malignant tumors 11 hodgkin lymphoma ,39 non Hodgkin lymphoma) and 10(16.7%) samples were benign tumors. The patient’s age ranged between 7month and 80 years with mean 40 years, most patients 33 (55%) were more than 40 years and the remaining 27were less than40 years representing (45%) patients were more than 40 years. The majority of patients were males and the male: female ratio was 2.3:1 representing 42(70%) and the remaining 18(30%) were females. One section of 3µm thickness was cut from each paraffin block by rotary microtome and stained by immunohistochemical method (modified new indirect method) for detection of EBV. Data was collected from patients files and the obtained results were analyzed using SPSS computer program. Immunohistochemical detection of EBV was revealed positive result in 3/50 samples and negative result in 47/50 in malignant samples while all benign tumors gave negative result for EBV, with insignificant statistical association between EBV expression and histopathology diagnosis (P=0.427). This study concludes that there is no association between EBV detection and lymphoma.