Identification of an active component in Guiera Senegalensis plant Used for healing diabetes wounds

Abstract

The Africanspecies Ghobash(Guiera senegalensisJ. F. Gmel),is a small herb found mainly in West Africa. Its widespread use in traditional medicine. The aim of this study is to identify the bioactive ingredient responsible of healing diabetes wound. The aim were obtained using different process, First plant crude oil were extracted using the soxhlet apparatus and two organic solvent (Chloroform &Methanol). Then the natural product present in dry plant leave were investigate by phytochemical screening analysis tests which conform the main classes of secondary metabolites, namely terpenoids, alkaloids, flavonoids and stroides. The anti-bacterial activity test was carried out to show the bioactivities properties of the plant. The tests were positive in two types of bacteria (staphylococcus aureus& pseudomonas aeruginosa). The (GC/MS) analysis were carried out to separate and identify the components of the plant and their structures. The obtained results showed the presence of different active ingredients such as: flavonoids, alkaloids and steroids in high percentage. The expected component responsible of healing diabetes wound were suggested to be stigmasterol.