Immunohistochemical Detection of CK5/6 in Bladder Tumors among Sudanese Patients

Abstract

This is an analytical retrospective case control hospital based study conducted in Ibn Sena hospital in Khartoum state, during the period from April to August 2016. The study aimed to detect CK5/6 expression in bladder tumors using immunohistochemistry. Forty paraffin embedded blocks previously diagnosed as bladder tumors were collected. Samples include 30(75%) malignant tumors, (including transitional cell carcinoma 28(70%), adenocarcinoma 2(5%) samples) and 10(25%) bengin samples. The patientʼs age ranged between 49 and 70 years with mean age of 59 years, most patients were more than 55 years representing 22(55 %) and the remaining 18(45%) patients were less than 55 years. One section of 3micrometer thickness was cut from each paraffin block by rotary microtome and stained by immunohistochemical method (indirect streptoavidin-biotin immunoperoxidase technique) for detection of CK5/6. Data collected from patients files and results obtained were analyzed using SPSS computer program. Immunohistochemical expression of CK5/6 was revealed positive result in 20/30 samples and negative result in 10/30 samples in malignant tumors, while all benign tumors gave positive result for CK5/6 (10/10), with significant statistical association between CK5/6 expression and histopathology diagnosis (P =0.035). This study concludes that CK5/6 expression is associated with bengin forms of bladder tumors.