Determination of Antibiogram of Bacteria Isolated from Hospitals’ Curtains in Khartoum State

Abstract

Privacy curtains that separate patients care area in hospital may play an important role in the transmission of healthcare-associated pathogenic microorganisms. This study was carried out during the period from March to June 2015 to determine the antibiogram of bacteria isolated from hospital curtains. Atotal of (n=27) bacterial isolates were obtained from the Research Laboratory, Sudan University of Science and Technology. Purity of the isolates was checked by streaking on nutrient agar and examined microscopically. Gram stain and biochemical tests were used to confirm the identification of the isolates. Modified Kirby-Bauer disk diffusion technique was used to determine the antibiogram of isolates against traditionally used antibiotics. The antibiotics used were Gentamicin, Ciprofloxacin, Azithromycin, Imipenem, Cotrimoxazole, Vancomycin, Novobiocin, Methicillin, Tetracycline and Azithromycin. The re-identified isolates (n=27) were Bacillus species 11 (40.7%), Pseudomonas aeruoginosa 6 (22.2%), Staphylococcus aureus 5 (18.5%), Klebsiella pneumoniae 4 (14.8%), Staphylococccus epidemidis 1 (3.7%). The result of susceptibility of bacterial isolates revealed that all of the isolates were susceptible (100%) to Ciprofloxacin. Activites of other antibiotics ranged from 0.0% to 80%. Determination of susceptibility of each isolate to same antibiotics was found as follows: S. aureus (n=5) were susceptible (100%) to Ciprofloxacin, Amoxicillin, Gentamicin, Imipenem, Cotrimoxazole, Tetracycline and Novobiocin. (60%) susceptibility to Azithromycin and (40%) to Methicillin. S. epidermidis (n=1) was susceptible (100%) to Ciprofloxacin, Methicillin, Imipenem, Cotrimoxazole and Vancomycin. Klebsiella pneumoniae (n=4) were susceptible (100%) to Ciprofloxacin, Azithromycin, Cotrimoxazole and Novobiocin. (75%) susceptibility to Gentamicin. (50%) susceptibility to Amoxicillin, Methicillin, Vancomycin and (25%) to Imipenem. Pseudomonas aeruginosa (n=6) were susceptible (100%) to Ciprofloxacin, Gentamicin, Azithromycin, Imipenem and Vancomycin. (83.3%) to Amoxicillin. (50%) susceptibility to Tetracycline and Novobiocin. (33.3%) to Cotrimoxazole and (16.6%) to Methicillin. The study concluded that all isolates recovered from hospital curtains exhibited high susceptibility to all antibiotics examined . Further studies with more bacterial isolates and advanced techniques are requried to validate the results of this study.